Journal: Synthetic and Systems Biotechnology

Article Title: Modifying the upstream open reading frames of cellulase gene enhances cellulase production in Penicillium oxalicum

doi: 10.1016/j.synbio.2025.12.016

Figure Lengend Snippet: Effects of functional uORFs in the 5′-UTR of the eg1 gene on gene expression in P . oxalicum grown on Avicel. (a) RT-qPCR analysis of gfp transcription. (b) RT-qPCR assay of eg1 transcription in OE- eg1 - muORF mutants. (c) mRNA stability of eg1 following 6 h dactinomycin pretreatment. (d) mRNA stability of eg1 following 2 h cycloheximide pretreatment. Data values represent mean ± standard deviation. Each experiment was performed with at least three biological replicates. ∗ p < 0.05 and ∗∗ p < 0.01 denote statistically significant differences compared to the control strain eg1 -5′-UTR- GFP or OE eg1 .

Article Snippet: The medium was supplemented with either dactinomycin (Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China) at a final concentration of 5 μg/mL or cycloheximide (MedChemExpress, Monmouth Junction, NJ, USA) at a final concentration of 10 μg/mL.

Techniques: Functional Assay, Gene Expression, Quantitative RT-PCR, Standard Deviation, Control

Journal: Translational Vision Science & Technology

Article Title: Pathogenicity and Functional Analysis of Multi-Variant Allele of RPE 65 Causing Retinitis Pigmentosa

doi: 10.1167/tvst.15.2.1

Figure Lengend Snippet: Pathogenic variant damaged the stability of RPE65 possibly through the ubiquitination–proteasome pathway. ( A ) When HEK293T cells were transfected with WT variant plasmids for 24 hours and treated with 100-µM CHX, RPE65 expression was detected. ( B ) RPE65 stability was detected after cells were treated with 30-µM MG-132. ( C ) Twenty-four hours after transfection, WT mutant proteins were enriched by immunoprecipitation, and their ubiquitination levels were detected.

Article Snippet: After 24 hours of transfection, HEK293T cells were treated with 100-μM cycloheximide (CHX, HY-N0901; MedChemExpress, Monmouth Junction, NJ) or CHX mixed with MG-132 proteasome inhibitor (HY-12320; MedChemExpress).

Techniques: Variant Assay, Ubiquitin Proteomics, Transfection, Expressing, Mutagenesis, Immunoprecipitation

Journal: Translational Vision Science & Technology

Article Title: Pathogenicity and Functional Analysis of Multi-Variant Allele of RPE 65 Causing Retinitis Pigmentosa

doi: 10.1167/tvst.15.2.1

Figure Lengend Snippet: Pathogenic variant damaged the stability of RPE65 possibly through the ubiquitination–proteasome pathway. ( A ) When HEK293T cells were transfected with WT variant plasmids for 24 hours and treated with 100-µM CHX, RPE65 expression was detected. ( B ) RPE65 stability was detected after cells were treated with 30-µM MG-132. ( C ) Twenty-four hours after transfection, WT mutant proteins were enriched by immunoprecipitation, and their ubiquitination levels were detected.

Article Snippet: After 24 hours of transfection, HEK293T cells were treated with 100-μM cycloheximide (CHX, HY-N0901; MedChemExpress, Monmouth Junction, NJ) or CHX mixed with MG-132 proteasome inhibitor (HY-12320; MedChemExpress).

Techniques: Variant Assay, Ubiquitin Proteomics, Transfection, Expressing, Mutagenesis, Immunoprecipitation